Distinguishing between male tree frog and female tree frogs can be difficult. For every rule made to differentiate between sexes, an exception can be found in at least one species. For this reason, it’s best to research the specific species of tree frog you are interested in breeding to determine how to tell males apart from females. Some of the best ways to sex tree frogs are outlined below.
Tree Frog Calling
A good method to sex many species is to listen for their call. Mature male tree frogs vocalize to attract mates, so if a Tree frog calls it’s often a male. Tree Frog Females of some species may also vocalize in response to the call of a male, making it difficult to distinguish males from females among these particular tree frogs. Both sexes will also emit distress calls when they are threatened, but this type of vocalization is not as pronounced as the loud advertisement call from a male. Tree Frogs that have been calling for extended periods of time often have a dark or baggy-looking throat, so by examining it, you can often tell which tree frogs have been calling and which frogs have not.
Tree Frog Size
Adult female tree frogs are usually larger than males. In some species, females may be almost twice as large as males, while in others the size difference may be more subtle. In addition, females often have a slightly broader head or more robust appearance than males. Individual animals can vary a great deal in size and shape, with large males appearing to be females, and small or young females looking like males. The body structure and size of a tree frog are best used to sex them in addition to other more reliable methods.
Tree Frog Nuptial Pads
Nuptial pads are found on male tree frogs. They appear at the base of the thumb during breeding season, and are used to help grasp female tree frogs while in amplexus (the mating position). Usually, nuptial pads are dark in color and look rough in appearance when compared to the rest of the skin. They can be hard to see in some species, while in others they are fairly prominent. Nuptial pads only develop in male tree frogs during the breeding season or when males are conditioned to breed in captivity, but if they are present, the frog is unquestionably male.